rabbit polyclonal anti sm mhc antibody  (Biomedical Technologies)

Journal: Biochemistry and Biophysics Reports

Article Title: The role of Smoothelin-B in abdominal aortic aneurysm formation

doi: 10.1016/j.bbrep.2026.102631

Figure Lengend Snippet: Comparison of mRNA expression levels of SM-MHC (A), α-SMA (B), SM-calponin (C), S100A4 (D), F4/80 (E), IL-6 (F), MCP-1 (G), Ccl5 (H), TGF-β (I), and Col1a1 (J) between SKO mice with AAA and DKO mice without aneurysm formation. Real-time PCR shows no significant differences in the mRNA expression levels of α-SMA , SM-MHC , or SM-calponin between the two groups. The mRNA expression levels of S100A4 , F4/80 , MCP-1 , Ccl5 , TGF-β and Col1a1 were significantly lower in the DKO group, without aneurysm formation, than in the SKO group, with AAA. Each data point represents an individual mouse (biological replicate). The numbers of analyzed samples were as follows: SM-MHC (SKO n = 8, DKO n = 7), α-SMA (SKO n = 7, DKO n = 7), SM-calponin (SKO n = 8, DKO n = 7), S100A4 (SKO n = 8, DKO n = 7), F4/80 (SKO n = 6, DKO n = 7), IL-6 (SKO n = 7, DKO n = 7), MCP-1 (SKO n = 8, DKO n = 7), Ccl5 (SKO n = 7, DKO n = 7), TGF-β (SKO n = 8, DKO n = 7), and Col1a1 (SKO n = 7, DKO n = 7). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant in Kruskal-Wallis test.

Article Snippet: Immunohistochemical analyses were carried out using a primary rabbit polyclonal anti-SM-MHC antibody (Biomedical Technologies Inc., Madrid, Spain; dilution 1:400) and a primary rat monoclonal anti-Mac-2 antibody (clone M3/38, Cedarlane, Ontario, Canada; dilution 1:3000).

Techniques: Comparison, Expressing, Real-time Polymerase Chain Reaction

Journal: Biochemistry and Biophysics Reports

Article Title: The role of Smoothelin-B in abdominal aortic aneurysm formation

doi: 10.1016/j.bbrep.2026.102631

Figure Lengend Snippet: Quantitative histopathological and immunohistochemical analysis of aortic tissues. Representative images of Alcian blue staining (A, B), SM-MHC immunostaining (D, E), Mac-2 immunostaining (G, H), S100A4 immunostaining (J, K), MCP-1 immunostaining (M, N), and Ccl5 immunostaining (P, Q) in the SKO and DKO groups. Representative images from the SKO and DKO groups are shown in the upper and middle rows, respectively, and the corresponding quantitative analyses are shown in the bottom row. The corresponding quantitative analyses are shown in panels C, F, I, L, O, and R, respectively. The stained areas were quantified using ImageJ. For Alcian blue, SM-MHC, S100A4, MCP-1, and Ccl5 staining, the positive staining areas were normalized to the medial area of the aortic wall. For Mac-2 staining, the positive area was normalized to the combined intimal and medial area because macrophage infiltration was frequently observed in both layers. Each dot represents the value obtained from a single analyzed section. Sections containing advanced aneurysmal lesions were excluded to avoid secondary changes, and samples with inadequate tissue orientation were also excluded from the analysis. The numbers of analyzed sections were as follows: Alcian blue staining (SKO n = 21, DKO n = 39), SM-MHC staining (SKO n = 20, DKO n = 35), Mac-2 staining (SKO n = 29, DKO n = 37), S100A4 staining (SKO n = 15, DKO n = 15), MCP-1 staining (SKO n = 14, DKO n = 16), and Ccl5 staining (SKO n = 14, DKO n = 16). Statistical comparisons between groups were performed using the Kruskal–Wallis test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; ns, not significant.

Article Snippet: Immunohistochemical analyses were carried out using a primary rabbit polyclonal anti-SM-MHC antibody (Biomedical Technologies Inc., Madrid, Spain; dilution 1:400) and a primary rat monoclonal anti-Mac-2 antibody (clone M3/38, Cedarlane, Ontario, Canada; dilution 1:3000).

Techniques: Immunohistochemical staining, Staining, Immunostaining

Journal: CJC Open

Article Title: Relationship Between Vascular Smooth Muscle Cell Phenotype and Degeneration of Elastin in the Aortic Media in Patients With Acute Aortic Dissection

doi: 10.1016/j.cjco.2025.03.020

Figure Lengend Snippet: Immunohistochemistry and immunoblotting of vascular smooth muscle phenotypic markers in the aortic media. Hematoxylin-and-eosin–stained sections of the aortic media ( A ). Immunohistochemistry revealed a significantly decreased positive area of smooth muscle myosin heavy chain (SM-MHC) ( B ) and smoothelin ( C ) immunostaining, and an increased positive area for S100A4 immunostaining ( D ) in the dissected media compared with control aortic media. Immunoblotting revealed a tendency similar to that observed by immunohistochemistry ( E-G ). See for the original Western blotting images. Scale bars = 200 μm.

Article Snippet: Rabbit polyclonal antibodies against SM-MHC (#21404-1-AP; Proteintech, Rosemont, IL), S100A4 (#A5114; Agilent Technologies, Santa Clara, CA), and elastin (#15257-1-AP; Proteintech) were used.

Techniques: Immunohistochemistry, Western Blot, Staining, Immunostaining, Control

Journal: CJC Open

Article Title: Relationship Between Vascular Smooth Muscle Cell Phenotype and Degeneration of Elastin in the Aortic Media in Patients With Acute Aortic Dissection

doi: 10.1016/j.cjco.2025.03.020

Figure Lengend Snippet: The relationship between each protein expression level. The levels of 2 well-differentiated vascular smooth muscle cell (VSMC) markers, SM-MHC, and smoothelin, correlated well ( A ). S100A4 levels were negatively correlated with smoothelin ( B ) and SM-MHC ( C ) levels. A significant positive correlation was observed between the distribution of elastin and smoothelin ( D ), and SM-MHC ( E ) levels. A negative correlation was observed between elastin and S100A4 levels, but it was not statistically significant ( F ). ρ, correlation coefficient.

Article Snippet: Rabbit polyclonal antibodies against SM-MHC (#21404-1-AP; Proteintech, Rosemont, IL), S100A4 (#A5114; Agilent Technologies, Santa Clara, CA), and elastin (#15257-1-AP; Proteintech) were used.

Techniques: Expressing